Automated Synthesis of Carbohydrates

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Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography† †Electronic supplementary information (ESI) available: Additional figures and characterization of final compound. See DOI: 10.1039/c6cc07584c Click here for additional data file.

Given recent advances in automated oligosaccharide synthesis, analytical techniques that can be coupled to a synthetic framework are needed to not just identify but also purify to homogeneity protected carbohydrate compounds at levels of ≥99.5% purity. Herein, an alternate-pump recycling high-performance liquid chromatography (R-HPLC) method has been developed to allow purification of protected...

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تاریخ انتشار 2009